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MacBinary | 1996-09-22 | 47.8 KB | [ CWWP/BOBO]
open in: MacOS 8.1
extracted
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Win98
extracted
|
DOS
extracted
browse contents |
view JSON data
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view as text
This file was processed as: MacBinary
(archive/macBinary ).
Confidence Program Detection Match Type Support
100%
dexvert
ClarisWorks/AppleWorks Document (document/clarisWorks)
idMeta
Supported
10%
dexvert
MacBinary (archive/macBinary)
fallback
Supported
100%
file
MacBinary II, inited, Thu Mar 28 17:29:46 1996, modified Sun Sep 22 08:44:46 1996, creator Apple Works, type 'CWWP', 48316 bytes "Instructions" , at 0xbd3c 322 bytes resource
default (weak)
99%
file
data
default
83%
TrID
Claris Works text (MacBinary)
default
16%
TrID
MacBinary 2
default (weak)
100%
siegfried
fmt/1762 MacBinary (II)
default
100%
lsar
MacBinary
default
id metadata key value macFileType [ CWWP] macFileCreator [ BOBO]
hex view +--------+-------------------------+-------------------------+--------+--------+ |00000000| 00 0c 49 6e 73 74 72 75 | 63 74 69 6f 6e 73 00 00 |..Instru|ctions..| |00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000040| 00 43 57 57 50 42 4f 42 | 4f 01 00 00 00 00 00 00 |.CWWPBOB|O.......| |00000050| 00 00 00 00 00 bc bc 00 | 00 01 42 ad 80 c3 5a ae |........|..B...Z.| |00000060| 6a e5 3e 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |j.>.....|........| |00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 ad 3d 00 00 |........|.....=..| |00000080| 04 00 8b 00 42 4f 42 4f | 00 01 00 7e a6 b4 00 00 |....BOBO|...~....| |00000090| 00 00 03 4a 02 53 00 39 | 00 47 00 39 00 47 00 39 |...J.S.9|.G.9.G.9| |000000a0| 00 47 03 11 02 0c 01 00 | 00 01 00 00 00 00 00 00 |.G......|........| |000000b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 01 00 00 02 |........|........| |000000c0| 00 01 00 00 00 7e a3 e4 | 00 00 00 0c 00 00 00 09 |.....~..|........| |000000d0| 00 01 00 00 00 01 00 01 | 00 02 00 1c 58 b1 00 0a |........|....X...| |000000e0| 00 00 00 01 00 00 00 01 | 00 7e a3 e8 00 00 00 00 |........|.~......| |000000f0| 02 02 00 00 00 00 00 00 | 00 00 00 00 00 00 00 3e |........|.......>| |00000100| 00 01 00 00 00 82 00 00 | 00 00 00 00 04 02 06 02 |........|........| |00000110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000120| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000130| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000140| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000150| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000160| 00 00 00 0b 00 00 0d 85 | 00 03 00 00 0c 01 ff ff |........|........| |00000170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 7e a6 a8 |........|.....~..| |00000180| 01 01 ff ff 00 00 00 01 | 00 01 01 fe 02 00 01 00 |........|........| |00000190| 00 02 00 02 00 00 00 00 | 00 00 01 00 00 00 01 00 |........|........| |000001a0| 00 01 00 01 00 2d 01 00 | 01 00 01 00 00 00 00 5a |.....-..|.......Z| |000001b0| 00 00 00 12 00 00 00 00 | 00 06 01 00 00 00 00 01 |........|........| |000001c0| 00 01 00 00 00 0e 00 0d | 7f ff ff ff 00 00 00 00 |........|........| |000001d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |000001e0| 00 00 00 00 00 00 00 00 | 00 00 00 78 00 03 00 00 |........|...x....| |000001f0| 00 48 00 48 00 00 00 00 | 03 0d 02 1b ff e2 ff e4 |.H.H....|........| |00000200| 03 2b 02 37 03 47 05 7b | 03 e0 00 02 00 00 00 48 |.+.7.G.{|.......H| |00000210| 00 48 00 00 00 00 03 0d | 02 1b 00 01 00 00 00 64 |.H......|.......d| |00000220| 00 00 00 01 00 01 01 01 | 00 02 00 01 27 0f 00 01 |........|....'...| |00000230| 00 01 00 00 00 00 00 00 | 00 00 00 00 00 00 60 08 |........|......`.| |00000240| 00 19 01 90 00 00 00 00 | 30 00 00 00 00 00 00 00 |........|0.......| |00000250| 00 00 00 01 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000260| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 1c |........|........| |00000270| 00 00 ff ff 00 00 00 04 | 00 10 00 01 00 05 00 00 |........|........| |00000280| 01 00 01 01 00 00 01 00 | 00 00 00 00 00 00 00 00 |........|........| |00000290| 00 7f f6 94 01 00 00 00 | 00 01 00 04 00 00 00 00 |........|........| |000002a0| 00 00 00 00 00 00 00 00 | 00 00 00 02 00 00 00 01 |........|........| |000002b0| 00 0d 00 00 00 0d 00 1d | 00 00 fe 00 06 0f ff ff |........|........| |000002c0| 00 01 01 86 fe 00 04 03 | 03 0f d0 0d fe 00 fe 03 |........|........| |000002d0| 01 00 0d fb 00 00 00 00 | 02 5b 00 00 2b 2a 00 00 |........|.[..+*..| |000002e0| 00 2f 00 01 00 01 00 c0 | 01 eb df fe 00 06 00 00 |./......|........| |000002f0| 01 1c 00 ff fd fd 00 00 | 00 40 fd 00 fd 30 02 0d |........|.@...0..| |00000300| ff dd 00 00 2a 3d 20 31 | 09 00 00 df ff ff 00 7e |....*= 1|.......~| |00000310| a3 f0 00 00 00 00 00 00 | 00 00 00 7e a2 c0 00 00 |........|...~....| |00000320| 00 00 00 00 00 00 00 00 | 00 00 0f 00 fb 03 00 0d |........|........| |00000330| fe ff 00 0c 00 0c 00 0f | ff 31 02 00 00 0d 00 0e |........|.1......| |00000340| 00 47 00 1a 02 0c 00 00 | 0f ff fd 00 0a d0 00 0f |.G......|........| |00000350| ff ff d0 00 0d ff 00 0e | 00 47 00 1a 02 0c 00 fd |........|.G......| |00000360| 30 00 df fc ff 04 fd 00 | 00 04 65 2e fe 00 00 00 |0.......|..e.....| |00000370| 00 00 02 d0 03 03 fe 00 | 01 0f fd fb 00 00 00 00 |........|........| |00000380| fd 00 00 0f ff f0 fd 03 | 01 00 00 00 00 00 00 00 |........|........| |00000390| 00 00 00 7e a4 80 00 00 | 0f 72 00 00 00 00 01 1c |...~....|.r......| |000003a0| 01 eb 00 01 00 00 01 00 | 00 00 00 01 00 00 01 00 |........|........| |000003b0| 00 00 00 00 00 00 00 00 | 00 00 00 80 18 00 00 00 |........|........| |000003c0| 00 00 00 8f b5 e6 0b 00 | 00 00 00 00 00 00 00 0d |........|........| |000003d0| d0 00 00 0d fe 00 00 01 | 00 7e a6 30 ff d0 00 7e |........|.~.0...~| |000003e0| a3 f8 00 00 21 28 00 00 | 00 00 00 00 00 00 00 00 |....!(..|........| |000003f0| 00 00 ff ff ff ff ff ff | ff ff 00 28 00 03 01 53 |........|...(...S| |00000400| 01 fd 00 07 44 53 45 54 | 00 00 01 bc 00 0d 00 00 |....DSET|........| |00000410| 6b f9 00 00 0d 85 00 00 | 01 00 00 01 00 00 00 03 |k.......|........| |00000420| 00 00 6f cc 00 25 00 b0 | 02 1f 00 00 00 7e a3 c0 |..o..%..|.....~..| |00000430| 00 7e a3 c8 00 7e a3 c4 | 00 00 00 00 00 00 00 7e |.~...~..|.......~| |00000440| a3 28 00 00 00 00 01 c5 | 02 bb 00 7e a2 84 00 25 |.(......|...~...%| |00000450| 02 aa 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........| |00000460| 00 00 06 39 01 c5 02 bb | 00 7e a4 70 00 24 02 b4 |...9....|.~.p.$..| |00000470| 00 00 00 00 00 00 00 01 | 00 00 00 00 00 00 00 00 |........|........| |00000480| 10 12 01 c5 02 bb 00 7e | a1 cc 00 25 02 b4 00 00 |.......~|...%....| |00000490| 00 00 00 00 00 02 00 00 | 00 00 00 00 00 00 1a 6f |........|.......o| |000004a0| 01 c5 02 bb 00 7e a4 44 | 00 21 02 b0 00 00 00 00 |.....~.D|.!......| |000004b0| 00 00 00 03 00 00 00 00 | 00 00 00 00 21 a3 01 c5 |........|....!...| |000004c0| 02 bb 00 7e a4 7c 00 22 | 02 b6 00 00 00 00 00 00 |...~.|."|........| |000004d0| 00 04 00 00 00 00 00 00 | 00 00 29 d0 01 c5 02 bb |........|..).....| |000004e0| 00 7e a4 50 00 23 02 a8 | 00 00 00 00 00 00 00 05 |.~.P.#..|........| |000004f0| 00 00 00 00 00 00 00 00 | 33 5e 01 c5 02 bb 00 7e |........|3^.....~| |00000500| a5 78 00 24 02 a4 00 00 | 00 00 00 00 00 06 00 00 |.x.$....|........| |00000510| 00 00 00 00 00 00 3d 5f | 01 c5 02 bb 00 7e a1 c8 |......=_|.....~..| |00000520| 00 24 02 ae 00 00 00 00 | 00 00 00 07 00 00 00 00 |.$......|........| |00000530| 00 00 00 00 48 58 01 c5 | 02 bb 00 7e a4 2c 00 22 |....HX..|...~.,."| |00000540| 02 a2 00 00 00 00 00 00 | 00 08 00 00 00 00 00 00 |........|........| |00000550| 00 00 51 0d 01 c5 02 bb | 00 7e a2 00 00 21 02 ba |..Q.....|.~...!..| |00000560| 00 00 00 00 00 00 00 09 | 00 00 00 00 00 00 00 00 |........|........| |00000570| 58 ac 01 c5 02 bb 00 7e | a4 8c 00 24 02 b8 00 00 |X......~|...$....| |00000580| 00 00 00 00 00 0a 00 00 | 00 00 00 00 00 00 63 1f |........|......c.| |00000590| 01 c5 02 bb 00 7e a1 dc | 00 24 02 ae 00 00 00 00 |.....~..|.$......| |000005a0| 00 00 00 0b 00 00 00 00 | 00 00 00 00 6d 5f 01 c5 |........|....m_..| |000005b0| 02 bb 00 7e a4 a0 00 09 | 00 ae 00 00 00 00 00 00 |...~....|........| |000005c0| 00 0c 00 00 00 00 00 00 | 00 00 05 80 00 00 00 00 |........|........| |000005d0| 00 0b 00 00 00 00 00 10 | 00 0c 00 00 00 00 00 30 |........|.......0| |000005e0| 00 0d 00 00 00 00 00 31 | 00 0d 00 00 00 00 00 32 |.......1|.......2| |000005f0| 00 0d 00 00 00 00 00 47 | 00 0d 00 00 00 00 01 e2 |.......G|........| |00000600| 00 0d 00 00 00 00 03 6d | 00 0d 00 00 00 00 03 78 |.......m|.......x| |00000610| 00 0d 00 00 00 00 03 79 | 00 0e 00 00 00 00 03 a3 |.......y|........| |00000620| 00 0e 00 00 00 00 03 d8 | 00 0e 00 00 00 00 04 04 |........|........| |00000630| 00 0e 00 00 00 00 04 1a | 00 0e 00 00 00 00 04 2d |........|.......-| |00000640| 00 0e 00 00 00 00 04 44 | 00 0d 00 00 00 00 04 45 |.......D|.......E| |00000650| 00 0d 00 00 00 00 04 46 | 00 0d 00 00 00 00 04 51 |.......F|.......Q| |00000660| 00 0d 00 00 00 00 06 28 | 00 0e 00 00 00 00 06 29 |.......(|.......)| |00000670| 00 0e 00 00 00 00 06 2a | 00 0e 00 00 00 00 06 2b |.......*|.......+| |00000680| 00 0e 00 00 00 00 06 2c | 00 0d 00 00 00 00 06 39 |.......,|.......9| |00000690| 00 0d 00 00 00 00 0a 1a | 00 0d 00 00 00 00 0b f8 |........|........| |000006a0| 00 0d 00 00 00 00 0c 3e | 00 0f 00 00 00 00 0c c0 |.......>|........| |000006b0| 00 0d 00 00 00 00 0c fb | 00 0f 00 00 00 00 0d 86 |........|........| |000006c0| 00 0f 00 00 00 00 0d 87 | 00 0d 00 00 00 00 0d e8 |........|........| |000006d0| 00 0d 00 00 00 00 0d e9 | 00 0d 00 00 00 00 0d ea |........|........| |000006e0| 00 0d 00 00 00 00 0d f4 | 00 0d 00 00 00 00 0e 33 |........|.......3| |000006f0| 00 0d 00 00 00 00 0e 34 | 00 0d 00 00 00 00 0e 4b |.......4|.......K| |00000700| 00 0d 00 00 00 00 11 2f | 00 0d 00 00 00 00 12 c8 |......./|........| |00000710| 00 0d 00 00 00 00 12 c9 | 00 0d 00 00 00 00 12 e2 |........|........| |00000720| 00 0d 00 00 00 00 14 87 | 00 0d 00 00 00 00 14 88 |........|........| |00000730| 00 0d 00 00 00 00 14 96 | 00 0d 00 00 00 00 19 f7 |........|........| |00000740| 00 0d 00 00 00 00 1a 5f | 00 0d 00 00 00 00 1a 60 |......._|.......`| |00000750| 00 0d 00 00 00 00 1a 6f | 00 0d 00 00 00 00 1c 8f |.......o|........| |00000760| 00 0d 00 00 00 00 1c 90 | 00 0d 00 00 00 00 1c 9f |........|........| |00000770| 00 0d 00 00 00 00 1d 86 | 00 0d 00 00 00 00 1d 87 |........|........| |00000780| 00 0d 00 00 00 00 1d 8d | 00 0d 00 00 00 00 1e 21 |........|.......!| |00000790| 00 0d 00 00 00 00 1e 22 | 00 0d 00 00 00 00 1e 35 |......."|.......5| |000007a0| 00 0d 00 00 00 00 1f b7 | 00 0d 00 00 00 00 1f b8 |........|........| |000007b0| 00 0d 00 00 00 00 1f bd | 00 0d 00 00 00 00 20 32 |........|...... 2| |000007c0| 00 0d 00 00 00 00 20 33 | 00 0d 00 00 00 00 20 34 |...... 3|...... 4| |000007d0| 00 0d 00 00 00 00 20 3e | 00 0d 00 00 00 00 22 06 |...... >|......".| |000007e0| 00 0d 00 00 00 00 22 07 | 00 0d 00 00 00 00 22 13 |......".|......".| |000007f0| 00 0d 00 00 00 00 22 98 | 00 0d 00 00 00 00 23 20 |......".|......# | |00000800| 00 0d 00 00 00 00 24 ae | 00 0d 00 00 00 00 25 b0 |......$.|......%.| |00000810| 00 0d 00 00 00 00 25 b1 | 00 0d 00 00 00 00 25 b2 |......%.|......%.| |00000820| 00 0d 00 00 00 00 25 bd | 00 0d 00 00 00 00 27 19 |......%.|......'.| |00000830| 00 0d 00 00 00 00 27 1a | 00 0d 00 00 00 00 27 31 |......'.|......'1| |00000840| 00 0d 00 00 00 00 27 a3 | 00 0d 00 00 00 00 28 bc |......'.|......(.| |00000850| 00 0d 00 00 00 00 2a e2 | 00 0d 00 00 00 00 2b 8d |......*.|......+.| |00000860| 00 0d 00 00 00 00 2b 8e | 00 0d 00 00 00 00 2b aa |......+.|......+.| |00000870| 00 0d 00 00 00 00 2d 2f | 00 0d 00 00 00 00 2d ea |......-/|......-.| |00000880| 00 0d 00 00 00 00 2f d0 | 00 0d 00 00 00 00 2f d1 |....../.|....../.| |00000890| 00 0d 00 00 00 00 2f d7 | 00 0d 00 00 00 00 30 5f |....../.|......0_| |000008a0| 00 0d 00 00 00 00 31 b7 | 00 0d 00 00 00 00 32 92 |......1.|......2.| |000008b0| 00 0d 00 00 00 00 33 5e | 00 0d 00 00 00 00 34 aa |......3^|......4.| |000008c0| 00 0d 00 00 00 00 34 ab | 00 0d 00 00 00 00 34 ba |......4.|......4.| |000008d0| 00 10 00 00 00 00 36 ff | 00 0d 00 00 00 00 37 00 |......6.|......7.| |000008e0| 00 0d 00 00 00 00 37 0e | 00 10 00 00 00 00 39 9d |......7.|......9.| |000008f0| 00 0d 00 00 00 00 39 9e | 00 0d 00 00 00 00 39 aa |......9.|......9.| |00000900| 00 0d 00 00 00 00 3a 51 | 00 0d 00 00 00 00 3c 68 |......:Q|......<h| |00000910| 00 0d 00 00 00 00 3c c9 | 00 0d 00 00 00 00 3d 5f |......<.|......=_| |00000920| 00 0d 00 00 00 00 3e 5a | 00 0d 00 00 00 00 3f 59 |......>Z|......?Y| |00000930| 00 0d 00 00 00 00 3f 5a | 00 0d 00 00 00 00 3f 6d |......?Z|......?m| |00000940| 00 0d 00 00 00 00 40 6a | 00 0d 00 00 00 00 42 79 |......@j|......By| |00000950| 00 0d 00 00 00 00 43 29 | 00 0d 00 00 00 00 44 d1 |......C)|......D.| |00000960| 00 0d 00 00 00 00 45 87 | 00 0d 00 00 00 00 45 88 |......E.|......E.| |00000970| 00 0d 00 00 00 00 45 99 | 00 0d 00 00 00 00 48 99 |......E.|......H.| |00000980| 00 0d 00 00 00 00 49 e8 | 00 0d 00 00 00 00 49 e9 |......I.|......I.| |00000990| 00 0d 00 00 00 00 49 f7 | 00 0d 00 00 00 00 4b a4 |......I.|......K.| |000009a0| 00 0d 00 00 00 00 4b a5 | 00 0d 00 00 00 00 4b ac |......K.|......K.| |000009b0| 00 0d 00 00 00 00 4c 69 | 00 0d 00 00 00 00 4c 6a |......Li|......Lj| |000009c0| 00 0d 00 00 00 00 4c 72 | 00 0d 00 00 00 00 4f 0b |......Lr|......O.| |000009d0| 00 0d 00 00 00 00 50 2a | 00 0d 00 00 00 00 51 0c |......P*|......Q.| |000009e0| 00 0d 00 00 00 00 51 0d | 00 0d 00 00 00 00 51 20 |......Q.|......Q | |000009f0| 00 0d 00 00 00 00 51 a7 | 00 0d 00 00 00 00 52 43 |......Q.|......RC| |00000a00| 00 0d 00 00 00 00 53 b1 | 00 0d 00 00 00 00 53 b2 |......S.|......S.| |00000a10| 00 0d 00 00 00 00 53 c6 | 00 0d 00 00 00 00 56 f2 |......S.|......V.| |00000a20| 00 0d 00 00 00 00 56 f3 | 00 0d 00 00 00 00 57 01 |......V.|......W.| |00000a30| 00 0d 00 00 00 00 57 b9 | 00 0d 00 00 00 00 57 f0 |......W.|......W.| |00000a40| 00 0d 00 00 00 00 58 03 | 00 0d 00 00 00 00 58 14 |......X.|......X.| |00000a50| 00 0d 00 00 00 00 58 57 | 00 0d 00 00 00 00 58 8b |......XW|......X.| |00000a60| 00 0d 00 00 00 00 58 aa | 00 0d 00 00 00 00 58 ab |......X.|......X.| |00000a70| 00 0d 00 00 00 00 58 ac | 00 0d 00 00 00 00 58 b9 |......X.|......X.| |00000a80| 00 0d 00 00 00 00 59 bc | 00 0d 00 00 00 00 59 bd |......Y.|......Y.| |00000a90| 00 0d 00 00 00 00 59 c8 | 00 0d 00 00 00 00 5b 85 |......Y.|......[.| |00000aa0| 00 0d 00 00 00 00 5c 1c | 00 0d 00 00 00 00 5c 1d |......\.|......\.| |00000ab0| 00 0d 00 00 00 00 5c 29 | 00 0d 00 00 00 00 5c 7e |......\)|......\~| |00000ac0| 00 0d 00 00 00 00 5e f8 | 00 0d 00 00 00 00 60 7c |......^.|......`|| |00000ad0| 00 0d 00 00 00 00 62 62 | 00 0d 00 00 00 00 64 95 |......bb|......d.| |00000ae0| 00 0d 00 00 00 00 66 66 | 00 0d 00 00 00 00 69 16 |......ff|......i.| |00000af0| 00 0d 00 00 00 00 69 17 | 00 0d 00 00 00 00 69 26 |......i.|......i&| |00000b00| 00 0d 00 00 00 00 6a 60 | 00 0d 00 00 00 00 6a 61 |......j`|......ja| |00000b10| 00 0d 00 00 00 00 6a 76 | 00 0d 00 00 00 00 6c e9 |......jv|......l.| |00000b20| 00 0d 00 00 00 00 6c ea | 00 0d 00 00 00 00 6c fe |......l.|......l.| |00000b30| 00 0d 00 00 00 00 6e 75 | 00 0d 00 00 00 00 6e 76 |......nu|......nv| |00000b40| 00 0d 00 00 00 00 6e 8b | 00 0d 00 00 00 00 19 74 |......n.|.......t| |00000b50| 00 00 00 00 00 02 00 01 | 12 c2 00 1a 00 00 00 0f |........|........| |00000b60| 00 01 00 00 0c 01 00 1b | 00 00 00 10 00 03 00 00 |........|........| |00000b70| 0a 01 00 1d 00 00 00 2f | 00 01 00 00 0c 01 00 1e |......./|........| |00000b80| 00 00 00 30 00 01 00 00 | 0c 01 00 1f 00 00 00 31 |...0....|.......1| |00000b90| 00 03 00 00 0c 01 00 20 | 00 00 00 32 00 00 00 01 |....... |...2....| |00000ba0| 0e 2f 00 23 00 00 00 47 | 00 03 00 00 0c 01 00 20 |./.#...G|....... | |00000bb0| 00 00 03 79 00 03 00 00 | 0c 01 00 24 00 00 04 44 |...y....|...$...D| |00000bc0| 00 03 00 00 0c 01 00 20 | 00 00 04 45 00 03 00 00 |....... |...E....| |00000bd0| 0c 01 00 20 00 00 04 46 | 00 00 00 01 0e 2f 00 23 |... ...F|...../.#| |00000be0| 00 00 04 51 00 03 00 00 | 0c 01 00 20 00 00 06 28 |...Q....|... ...(| |00000bf0| 00 03 00 00 0c 01 00 24 | 00 00 06 2c 00 00 00 01 |.......$|...,....| |00000c00| 0e 2f 00 23 00 00 06 38 | 00 03 00 00 0c 01 00 20 |./.#...8|....... | |00000c10| 00 00 0b f8 00 03 00 01 | 0c 01 00 25 00 00 0c 04 |........|...%....| |00000c20| 00 03 00 00 0c 01 00 20 | 00 00 0c 3e 00 03 00 00 |....... |...>....| |00000c30| 0c 01 00 26 00 00 0c 9b | 00 03 00 02 0c 01 00 27 |...&....|.......'| |00000c40| 00 00 0c b2 00 03 00 00 | 0c 01 00 26 00 00 0c b3 |........|...&....| |00000c50| 00 03 00 01 0c 01 00 28 | 00 00 0c b5 00 03 00 00 |.......(|........| |00000c60| 0c 01 00 26 00 00 0c c0 | 00 03 00 01 0c 01 00 25 |...&....|.......%| |00000c70| 00 00 0c cb 00 03 00 00 | 0c 01 00 20 00 00 0c fa |........|... ....| |00000c80| 00 03 00 01 0c 01 00 25 | 00 00 0c fb 00 03 00 00 |.......%|........| |00000c90| 0c 01 00 26 00 00 0d 6c | 00 03 00 02 0c 01 00 16 |...&...l|........| |00000ca0| 00 00 0d 7c 00 03 00 00 | 0c 01 00 17 00 00 0d 85 |...|....|........| |00000cb0| 00 03 00 00 0c 01 00 26 | 00 00 0d 87 00 03 00 00 |.......&|........| |00000cc0| 0c 01 00 20 00 00 0d ea | 00 00 00 01 0e 2f 00 23 |... ....|...../.#| |00000cd0| 00 00 0d ef 00 04 00 01 | 0c 2f 00 2b 00 00 0d f3 |........|./.+....| |00000ce0| 00 03 00 00 0c 01 00 20 | 00 00 0e 34 00 00 00 01 |....... |...4....| |00000cf0| 0c d9 00 2c 00 00 0e 38 | 00 00 00 01 0c d9 00 2c |...,...8|.......,| |00000d00| 00 00 0e 4a 00 03 00 00 | 0c 01 00 20 00 00 0f 45 |...J....|... ...E| |00000d10| 00 03 00 04 0c 01 00 2d | 00 00 0f 56 00 03 00 00 |.......-|...V....| |00000d20| 0c 01 00 20 00 00 0f 8e | 00 04 00 00 0c 01 00 29 |... ....|.......)| |00000d30| 00 00 0f a6 00 03 00 00 | 0c 01 00 20 00 00 12 c9 |........|... ....| |00000d40| 00 00 00 01 0c d9 00 2c | 00 00 12 e2 00 03 00 00 |.......,|........| |00000d50| 0c 01 00 20 00 00 13 bb | 00 04 00 00 0c 01 00 29 |... ....|.......)| |00000d60| 00 00 13 c1 00 03 00 00 | 0c 01 00 20 00 00 14 88 |........|... ....| |00000d70| 00 00 00 01 0c d9 00 2c | 00 00 14 8c 00 00 00 01 |.......,|........| |00000d80| 0c d9 00 2c 00 00 14 95 | 00 03 00 00 0c 01 00 20 |...,....|....... | |00000d90| 00 00 17 dd 00 04 00 00 | 0c 01 00 29 00 00 17 e1 |........|...)....| |00000da0| 00 03 00 00 0c 01 00 20 | 00 00 18 75 00 03 00 04 |....... |...u....| |00000db0| 0c 01 00 2d 00 00 18 7f | 00 03 00 00 0c 01 00 20 |...-....|....... | |00000dc0| 00 00 18 99 00 03 00 04 | 0c 01 00 2d 00 00 18 a1 |........|...-....| |00000dd0| 00 03 00 00 0c 01 00 20 | 00 00 19 47 00 04 00 00 |....... |...G....| |00000de0| 0c 01 00 29 00 00 19 52 | 00 03 00 00 0c 01 00 20 |...)...R|....... | |00000df0| 00 00 1a 60 00 00 00 01 | 0c d9 00 2c 00 00 1a 6f |...`....|...,...o| |00000e00| 00 03 00 00 0c 01 00 20 | 00 00 1a fb 00 04 00 00 |....... |........| |00000e10| 0c 01 00 29 00 00 1a ff | 00 03 00 00 0c 01 00 20 |...)....|....... | |00000e20| 00 00 1b 17 00 04 00 00 | 0c 01 00 29 00 00 1b 1e |........|...)....| |00000e30| 00 03 00 00 0c 01 00 20 | 00 00 1b ac 00 04 00 00 |....... |........| |00000e40| 0c 01 00 29 00 00 1b b8 | 00 03 00 00 0c 01 00 20 |...)....|....... | |00000e50| 00 00 1c 90 00 00 00 01 | 0c d9 00 2c 00 00 1c 9e |........|...,....| |00000e60| 00 00 00 01 0c d9 00 2c | 00 00 1c 9f 00 03 00 00 |.......,|........| |00000e70| 0c 01 00 20 00 00 1c ce | 00 04 00 00 0c 01 00 29 |... ....|.......)| |00000e80| 00 00 1c d5 00 03 00 00 | 0c 01 00 20 00 00 1d 87 |........|... ....| 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41 52 | 54 49 4e 47 20 4d 69 74 |)...STAR|TING Mit| |00002680| 6f 50 72 6f 74 20 49 49 | 0d 59 6f 75 20 6f 6e 6c |oProt II|.You onl| |00002690| 79 20 6e 65 65 64 20 74 | 6f 20 70 75 74 20 74 68 |y need t|o put th| |000026a0| 65 20 66 69 6c 65 20 4d | 69 74 6f 50 72 6f 74 20 |e file M|itoProt | |000026b0| 49 49 20 77 68 65 72 65 | 20 79 6f 75 20 77 61 6e |II where| you wan| |000026c0| 74 20 69 74 20 6f 6e 20 | 74 68 65 20 68 61 72 64 |t it on |the hard| |000026d0| 20 64 69 73 6b 2e 20 49 | 66 20 79 6f 75 20 68 61 | disk. 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Mi|toProt I| |000027e0| 49 20 63 61 6e 20 61 6c | 73 6f 20 62 65 20 69 6e |I can al|so be in| |000027f0| 73 74 61 6c 6c 65 64 20 | 6f 6e 20 52 2f 4f 20 64 |stalled |on R/O d| |00002800| 69 73 6b 73 2c 20 75 6e | 6c 69 6b 65 20 74 68 65 |isks, un|like the| |00002810| 20 70 72 65 63 65 64 65 | 6e 74 20 72 65 6c 65 61 | precede|nt relea| |00002820| 73 65 2e 0d 4f 6e 63 65 | 20 74 68 69 73 20 69 73 |se..Once| this is| |00002830| 20 64 6f 6e 65 2c 20 64 | 6f 75 62 6c 65 20 63 6c | done, d|ouble cl| |00002840| 69 63 6b 20 6f 6e 20 74 | 68 65 20 4d 69 74 6f 50 |ick on t|he MitoP| |00002850| 72 6f 74 20 69 63 6f 6e | 20 74 6f 20 73 74 61 72 |rot icon| to star| |00002860| 74 2e 20 57 68 65 6e 20 | 79 6f 75 20 6f 70 65 6e |t. 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If you| |000031f0| 20 75 73 65 20 69 74 20 | 74 6f 20 70 75 62 6c 69 | use it |to publi| |00003200| 73 68 20 61 6e 79 74 68 | 69 6e 67 2c 20 49 20 77 |sh anyth|ing, I w| |00003210| 6f 75 6c 64 20 62 65 20 | 70 6c 65 61 73 65 64 20 |ould be |pleased | |00003220| 69 66 20 79 6f 75 20 63 | 69 74 65 20 69 74 20 61 |if you c|ite it a| |00003230| 6d 6f 6e 67 20 79 6f 75 | 72 20 72 65 66 65 72 65 |mong you|r refere| |00003240| 6e 63 65 73 3a 0d 4d 69 | 74 6f 50 72 6f 74 20 31 |nces:.Mi|toProt 1| |00003250| 2e 30 20 5b 73 74 75 64 | 69 65 73 20 61 62 6f 75 |.0 [stud|ies abou| |00003260| 74 20 6d 69 74 6f 63 68 | 6f 6e 64 72 69 61 6c 20 |t mitoch|ondrial | |00003270| 74 61 72 67 65 74 69 6e | 67 20 73 65 71 75 65 6e |targetin|g sequen| |00003280| 63 65 73 20 28 4d 54 53 | 73 29 5d 0d 4d 2e 20 47 |ces (MTS|s)].M. G| |00003290| 2e 20 43 6c 61 72 6f 73 | 20 28 31 39 39 35 29 20 |. 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Tar|geting.M| |00004da0| 69 74 6f 50 72 6f 74 20 | 70 65 72 6d 69 74 73 20 |itoProt |permits | |00004db0| 74 68 65 20 75 73 65 72 | 20 74 6f 20 63 68 61 6e |the user| to chan| |00004dc0| 67 65 20 33 20 6f 66 20 | 74 68 65 20 70 61 72 61 |ge 3 of |the para| |00004dd0| 6d 65 74 65 72 73 20 74 | 68 61 74 20 61 72 65 20 |meters t|hat are | |00004de0| 6e 65 63 65 73 73 61 72 | 79 20 74 6f 20 61 73 73 |necessar|y to ass| |00004df0| 65 73 73 20 74 68 65 20 | 63 68 61 72 61 63 74 65 |ess the |characte| |00004e00| 72 69 73 74 69 63 73 20 | 6f 66 20 00 00 03 91 4d |ristics |of ....M| |00004e10| 54 53 73 3a 0d a5 20 53 | 63 61 6e 6e 69 6e 67 20 |TSs:.. S|canning | |00004e20| 57 69 6e 64 6f 77 20 69 | 73 20 74 68 65 20 73 69 |Window i|s the si| |00004e30| 7a 65 20 6f 66 20 61 20 | 6d 6f 76 69 6e 67 20 77 |ze of a |moving w| |00004e40| 69 6e 64 6f 77 20 77 68 | 69 63 68 20 70 65 72 6d |indow wh|ich perm| |00004e50| 69 74 73 20 63 61 6c 63 | 75 6c 61 74 69 6f 6e 20 |its calc|ulation | |00004e60| 6f 66 20 74 68 65 20 68 | 79 64 72 6f 70 68 6f 62 |of the h|ydrophob| |00004e70| 69 63 20 6d 6f 6d 65 6e | 74 20 28 b5 48 29 2c 20 |ic momen|t (.H), | |00004e80| 61 6e 64 20 72 65 76 65 | 61 6c 69 6e 67 20 74 68 |and reve|aling th| |00004e90| 65 20 6c 65 6e 67 74 68 | 20 6f 66 20 4d 54 53 73 |e length| of MTSs| |00004ea0| 2e 20 4f 70 74 69 6d 61 | 6c 20 72 65 73 75 6c 74 |. 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Foll|owing th| |00005040| 65 20 64 69 72 65 63 74 | 69 76 65 73 20 6f 66 20 |e direct|ives of | |00005050| 74 68 65 20 74 68 69 72 | 64 20 65 6e 74 72 79 20 |the thir|d entry | |00005060| 6f 66 20 70 72 65 66 65 | 72 65 6e 63 65 73 2c 20 |of prefe|rences, | |00005070| 74 68 65 20 45 6e 64 20 | 61 74 20 63 61 6e 20 62 |the End |at can b| |00005080| 65 20 74 68 65 20 64 65 | 66 61 75 6c 74 20 37 30 |e the de|fault 70| |00005090| 20 72 65 73 69 64 75 65 | 73 20 6f 72 20 74 68 65 | residue|s or the| |000050a0| 20 76 61 6c 75 65 20 6f | 62 74 61 69 6e 65 64 20 | value o|btained | |000050b0| 62 79 20 74 68 65 20 63 | 61 6c 63 75 6c 61 74 65 |by the c|alculate| |000050c0| 64 20 6d 61 78 69 6d 61 | 6c 20 73 65 71 75 65 6e |d maxima|l sequen| |000050d0| 63 65 20 70 6f 73 69 74 | 69 6f 6e 20 74 68 61 74 |ce posit|ion that| |000050e0| 20 63 61 6e 20 63 6f 6e | 74 61 69 6e 20 61 20 4d | can con|tain a M| |000050f0| 54 53 2e 20 45 6e 64 20 | 61 74 20 61 6c 77 61 79 |TS. 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To a|ssess hy| |00005340| 64 72 6f 70 68 6f 62 69 | 63 69 74 79 20 63 68 61 |drophobi|city cha| |00005350| 72 61 63 74 65 72 69 73 | 74 69 63 73 2c 20 33 20 |racteris|tics, 3 | |00005360| 70 61 72 61 6d 65 74 65 | 72 73 20 63 61 6e 20 62 |paramete|rs can b| |00005370| 65 20 6d 6f 64 69 66 69 | 65 64 2c 20 69 6e 20 61 |e modifi|ed, in a| |00005380| 64 64 69 74 69 6f 6e 20 | 74 6f 20 74 68 65 20 68 |ddition |to the h| |00005390| 79 64 72 6f 70 68 6f 62 | 69 63 69 74 79 20 73 63 |ydrophob|icity sc| |000053a0| 61 6c 65 3a 0d a5 20 53 | 63 61 6e 6e 69 6e 67 20 |ale:.. S|canning | |000053b0| 57 69 6e 64 6f 77 20 73 | 65 72 76 65 73 20 74 6f |Window s|erves to| |000053c0| 20 69 64 65 6e 74 69 66 | 79 20 74 68 65 20 66 72 | identif|y the fr| |000053d0| 61 67 6d 65 6e 74 20 6f | 66 20 68 69 67 68 65 73 |agment o|f highes| |000053e0| 74 20 68 79 64 72 6f 70 | 68 6f 62 69 63 69 74 79 |t hydrop|hobicity| |000053f0| 20 76 61 6c 75 65 20 28 | 48 31 37 2c 20 73 65 65 | value (|H17, see| |00005400| 20 62 65 6c 6f 77 29 2e | 20 41 20 72 61 6e 67 65 | below).| A range| |00005410| 20 6f 66 20 31 35 2d 32 | 31 20 72 65 73 69 64 75 | of 15-2|1 residu| |00005420| 65 73 20 63 61 6e 20 62 | 65 20 75 73 65 64 2c 20 |es can b|e used, | |00005430| 62 75 74 20 31 37 20 72 | 65 73 69 64 75 65 73 20 |but 17 r|esidues | |00005440| 75 73 75 61 6c 6c 79 20 | 70 72 6f 76 69 64 65 20 |usually |provide | |00005450| 70 72 6f 70 65 72 20 72 | 65 73 75 6c 74 73 2e 0d |proper r|esults..| |00005460| a5 20 53 6d 61 6c 6c 65 | 72 20 61 6e 64 20 42 69 |. 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They | |00005500| 73 68 6f 75 6c 64 20 6e | 6f 74 20 62 65 20 6d 6f |should n|ot be mo| |00005510| 64 69 66 69 65 64 20 73 | 69 6e 63 65 2c 20 64 69 |dified s|ince, di| |00005520| 6d 69 6e 69 73 68 69 6e | 67 20 74 68 65 6d 2c 20 |minishin|g them, | |00005530| 6d 65 73 6f 68 79 64 72 | 6f 70 68 6f 62 69 63 69 |mesohydr|ophobici| |00005540| 74 79 20 63 6f 6d 65 73 | 20 63 6c 6f 73 65 72 20 |ty comes| closer | |00005550| 74 6f 20 48 31 37 2c 20 | 00 00 03 0e 6f 72 20 69 |to H17, |....or i| |00005560| 66 20 69 6e 63 72 65 61 | 73 69 6e 67 20 74 68 65 |f increa|sing the| |00005570| 6d 2c 20 6d 65 73 6f 68 | 79 64 72 6f 70 68 6f 62 |m, mesoh|ydrophob| |00005580| 69 63 69 74 79 20 63 6f | 6d 65 73 20 63 6c 6f 73 |icity co|mes clos| |00005590| 65 72 20 74 6f 20 74 68 | 65 20 61 76 65 72 61 67 |er to th|e averag| |000055a0| 65 20 68 79 64 72 6f 70 | 68 6f 62 69 63 69 74 79 |e hydrop|hobicity| |000055b0| 2e 20 48 6f 77 65 76 65 | 72 2c 20 62 6f 74 68 20 |. 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This| |00005600| 20 69 73 20 77 68 79 20 | 73 65 71 75 65 6e 63 65 | is why |sequence| |00005610| 73 20 73 68 6f 72 74 65 | 72 20 74 68 61 6e 20 38 |s shorte|r than 8| |00005620| 30 20 72 65 73 69 64 75 | 65 73 20 73 68 6f 75 6c |0 residu|es shoul| |00005630| 64 20 62 65 20 74 72 65 | 61 74 65 64 20 73 65 70 |d be tre|ated sep| |00005640| 61 72 61 74 65 64 6c 79 | 2e 0d 0d 53 63 61 6c 65 |aratedly|...Scale| |00005650| 0d 4d 69 74 6f 50 72 6f | 74 20 70 72 6f 76 69 64 |.MitoPro|t provid| |00005660| 65 73 20 66 6f 75 72 20 | 64 69 66 66 65 72 65 6e |es four |differen| |00005670| 74 20 68 79 64 72 6f 70 | 68 6f 62 69 63 69 74 79 |t hydrop|hobicity| |00005680| 20 73 63 61 6c 65 73 20 | 74 6f 20 61 64 64 65 64 | scales |to added| |00005690| 20 66 6c 65 78 69 62 69 | 6c 69 74 79 2c 20 61 6e | flexibi|lity, an| |000056a0| 64 20 74 6f 20 65 6e 61 | 62 6c 65 20 63 6f 6d 70 |d to ena|ble comp| |000056b0| 61 72 69 73 6f 6e 20 6f | 66 20 72 65 73 75 6c 74 |arison o|f result| |000056c0| 73 20 77 69 74 68 20 64 | 69 66 66 65 72 65 6e 74 |s with d|ifferent| |000056d0| 20 73 63 61 6c 65 73 3a | 0d a5 20 47 45 53 2e 2d | scales:|.. GES.-| |000056e0| 20 45 6e 67 65 6c 6d 61 | 6e 2c 44 2e 4d 2e 2c 20 | Engelma|n,D.M., | |000056f0| 53 74 65 69 74 7a 2c 54 | 2e 41 2e 2c 20 47 6f 6c |Steitz,T|.A., Gol| |00005700| 64 6d 61 6e 2c 41 2e 20 | 28 31 39 38 36 29 20 41 |dman,A. |(1986) A| |00005710| 6e 6e 2e 20 52 65 76 2e | 20 42 69 6f 70 68 79 73 |nn. Rev.| Biophys| |00005720| 2e 20 42 69 6f 70 68 79 | 73 2e 20 43 68 65 6d 2e |. Biophy|s. Chem.| |00005730| 20 31 35 2c 20 33 32 31 | 2d 35 33 30 2e 20 54 68 | 15, 321|-530. Th| |00005740| 69 73 20 69 73 20 72 65 | 63 6f 6d 6d 65 6e 64 65 |is is re|commende| |00005750| 64 20 65 73 70 65 63 69 | 61 6c 6c 79 20 66 6f 72 |d especi|ally for| |00005760| 20 73 74 75 64 69 65 73 | 20 61 62 6f 75 74 20 68 | studies| about h| |00005770| 79 64 72 6f 70 68 6f 62 | 69 63 20 70 72 6f 74 65 |ydrophob|ic prote| |00005780| 69 6e 73 20 62 65 63 61 | 75 73 65 20 69 74 20 71 |ins beca|use it q| |00005790| 75 61 6e 74 69 66 69 65 | 73 20 74 68 65 20 66 72 |uantifie|s the fr| |000057a0| 65 65 20 65 6e 65 72 67 | 79 20 6f 66 20 77 61 74 |ee energ|y of wat| |000057b0| 65 72 2d 6f 69 6c 20 74 | 72 61 6e 73 66 65 72 20 |er-oil t|ransfer | |000057c0| 66 6f 72 20 72 65 73 69 | 64 75 65 73 20 69 6e 20 |for resi|dues in | |000057d0| 61 6e 20 61 2d 68 65 6c | 69 63 61 6c 20 73 74 72 |an a-hel|ical str| |000057e0| 75 63 74 75 72 65 2e 20 | 54 68 69 73 20 72 65 66 |ucture. |This ref| |000057f0| 6c 65 63 74 73 20 74 68 | 65 20 63 69 72 63 75 6d |lects th|e circum| |00005800| 73 74 61 6e 63 65 73 20 | 69 6e 20 77 68 69 63 68 |stances |in which| |00005810| 20 61 6d 69 6e 6f 20 61 | 63 69 64 73 20 61 70 70 | amino a|cids app| |00005820| 65 61 72 20 69 6e 20 70 | 72 6f 74 65 69 6e 73 2e |ear in p|roteins.| |00005830| 0d a5 20 47 76 48 31 2e | 2d 20 76 6f 6e 20 48 65 |.. GvH1.|- von He| |00005840| 69 6a 6e 65 2c 47 2e 20 | 28 31 39 39 32 29 20 4a |ijne,G. |(1992) J| |00005850| 2e 20 4d 6f 6c 2e 20 42 | 69 6f 6c 2e 20 32 32 35 |. Mol. B|iol. 225| |00005860| 2c 20 34 38 37 2d 39 34 | 2e 20 00 00 02 cf 54 68 |, 487-94|. ....Th| |00005870| 69 73 20 69 73 20 61 20 | 73 74 61 74 69 73 74 69 |is is a |statisti| |00005880| 63 61 6c 20 73 63 61 6c | 65 20 6f 62 74 61 69 6e |cal scal|e obtain| |00005890| 65 64 20 66 72 6f 6d 20 | 74 68 65 20 61 6d 69 6e |ed from |the amin| |000058a0| 6f 20 61 63 69 64 20 66 | 72 65 71 75 65 6e 63 69 |o acid f|requenci| |000058b0| 65 73 20 69 6e 20 74 68 | 65 20 63 65 6e 74 72 61 |es in th|e centra| |000058c0| 6c 20 70 61 72 74 20 6f | 66 20 61 20 74 72 61 6e |l part o|f a tran| |000058d0| 73 6d 65 6d 62 72 61 6e | 65 20 73 65 67 6d 65 6e |smembran|e segmen| |000058e0| 74 20 77 69 74 68 20 72 | 65 73 70 65 63 74 20 74 |t with r|espect t| |000058f0| 6f 20 74 68 65 20 6e 6f | 6e 2d 6d 65 6d 62 72 61 |o the no|n-membra| |00005900| 6e 6f 75 73 20 73 74 72 | 65 74 63 68 65 73 2e 0d |nous str|etches..| |00005910| a5 20 4b 44 2e 2d 20 4b | 79 74 65 2c 4a 2e 2c 20 |. KD.- K|yte,J., | |00005920| 44 6f 6f 6c 69 74 74 6c | 65 2c 52 2e 46 2e 20 28 |Doolittl|e,R.F. (| |00005930| 31 39 38 32 29 20 4a 2e | 20 4d 6f 6c 2e 20 42 69 |1982) J.| Mol. Bi| |00005940| 6f 6c 2e 20 31 35 37 2c | 20 31 30 35 2d 33 32 2e |ol. 157,| 105-32.| |00005950| 20 54 68 69 73 20 73 63 | 61 6c 65 20 74 61 6b 65 | This sc|ale take| |00005960| 73 20 69 6e 74 6f 20 61 | 63 63 6f 75 6e 74 20 76 |s into a|ccount v| |00005970| 61 6c 75 65 73 20 66 72 | 6f 6d 20 77 61 74 65 72 |alues fr|om water| |00005980| 2d 74 6f 2d 76 61 70 6f | 75 72 20 65 6e 65 72 67 |-to-vapo|ur energ| |00005990| 79 20 74 72 61 6e 73 66 | 65 72 73 20 61 6e 64 20 |y transf|ers and | |000059a0| 66 72 6f 6d 20 69 6e 74 | 65 72 6e 61 6c 2d 65 78 |from int|ernal-ex| |000059b0| 74 65 72 6e 61 6c 20 64 | 69 73 74 72 69 62 75 74 |ternal d|istribut| |000059c0| 69 6f 6e 20 6f 66 20 61 | 6d 69 6e 6f 20 61 63 69 |ion of a|mino aci| |000059d0| 64 20 72 65 73 69 64 75 | 65 73 2e 0d a5 20 45 43 |d residu|es... EC| |000059e0| 53 2e 2d 20 45 69 73 65 | 6e 62 65 72 67 2c 44 2e |S.- Eise|nberg,D.| |000059f0| 20 28 31 39 38 34 29 20 | 41 6e 6e 2e 20 52 65 76 | (1984) |Ann. Rev| |00005a00| 2e 20 42 69 6f 63 68 65 | 6d 2e 20 35 33 2c 20 35 |. Bioche|m. 53, 5| |00005a10| 39 35 2d 36 32 33 2e 20 | 54 68 65 20 d2 45 69 73 |95-623. |The .Eis| |00005a20| 65 6e 62 65 72 67 d5 73 | 20 63 6f 6e 73 65 6e 73 |enberg.s| consens| |00005a30| 75 73 20 53 63 61 6c 65 | d3 20 77 61 73 20 64 65 |us Scale|. was de| |00005a40| 73 69 67 6e 65 64 20 74 | 6f 20 6d 69 74 69 67 61 |signed t|o mitiga| |00005a50| 74 65 20 74 68 65 20 65 | 66 66 65 63 74 73 20 6f |te the e|ffects o| |00005a60| 66 20 6f 75 74 6c 61 79 | 69 6e 67 20 76 61 6c 75 |f outlay|ing valu| |00005a70| 65 73 20 69 6e 20 61 6e | 79 20 6f 6e 65 20 73 63 |es in an|y one sc| |00005a80| 61 6c 65 20 70 72 6f 64 | 75 63 65 64 20 62 79 20 |ale prod|uced by | |00005a90| 74 68 65 20 70 65 63 75 | 6c 69 61 72 69 74 69 65 |the pecu|liaritie| |00005aa0| 73 20 6f 66 20 74 68 65 | 20 6d 65 74 68 6f 64 2c |s of the| method,| |00005ab0| 20 61 6e 64 20 69 73 20 | 73 69 6d 70 6c 79 20 61 | and is |simply a| |00005ac0| 6e 20 61 76 65 72 61 67 | 65 20 6f 66 20 66 6f 75 |n averag|e of fou| |00005ad0| 72 20 73 63 61 6c 65 73 | 2e 20 49 74 20 67 69 76 |r scales|. It giv| |00005ae0| 65 73 20 62 65 74 74 65 | 72 20 72 65 73 75 6c 74 |es bette|r result| |00005af0| 73 20 74 68 61 6e 20 74 | 68 65 20 4f 4d 48 20 70 |s than t|he OMH p| |00005b00| 72 65 73 65 6e 74 65 64 | 20 69 6e 20 6f 6c 64 65 |resented| in olde| |00005b10| 72 20 76 65 72 73 69 6f | 6e 73 20 6f 66 20 4d 69 |r versio|ns of Mi| |00005b20| 74 6f 50 72 6f 74 2e 0d | 0d 54 61 72 67 65 74 69 |toProt..|.Targeti| |00005b30| 6e 67 20 7a 6f 6e 65 0d | 4d 69 74 6f 50 00 00 03 |ng zone.|MitoP...| |00005b40| 28 72 6f 74 20 63 61 6c | 63 75 6c 61 74 65 73 20 |(rot cal|culates | |00005b50| 74 68 65 20 4e 2d 74 65 | 72 6d 69 6e 61 6c 20 70 |the N-te|rminal p| |00005b60| 72 6f 74 65 69 6e 20 72 | 65 67 69 6f 6e 20 74 68 |rotein r|egion th| |00005b70| 61 74 20 63 61 6e 20 73 | 75 70 70 6f 72 74 20 61 |at can s|upport a| |00005b80| 6e 20 4d 54 53 20 61 6e | 64 20 74 68 65 20 63 6c |n MTS an|d the cl| |00005b90| 65 61 76 61 67 65 20 73 | 69 74 65 2e 20 54 68 75 |eavage s|ite. Thu| |00005ba0| 73 2c 20 69 74 20 73 65 | 61 72 63 68 65 73 20 77 |s, it se|arches w| |00005bb0| 69 74 68 69 6e 20 74 68 | 65 20 77 69 6e 64 6f 77 |ithin th|e window| |00005bc0| 20 64 65 74 65 72 6d 69 | 6e 65 64 20 62 79 20 53 | determi|ned by S| |00005bd0| 74 61 72 74 20 61 74 20 | 61 6e 64 20 45 6e 64 20 |tart at |and End | |00005be0| 61 74 20 70 61 72 61 6d | 65 74 65 72 73 20 66 6f |at param|eters fo| |00005bf0| 72 20 74 68 65 20 65 78 | 69 73 74 65 6e 63 65 20 |r the ex|istence | |00005c00| 6f 66 20 74 77 6f 20 63 | 6c 6f 73 65 20 47 6c 75 |of two c|lose Glu| |00005c10| 20 6f 72 20 41 73 70 20 | 77 69 74 68 69 6e 20 61 | or Asp |within a| |00005c20| 20 64 65 74 65 72 6d 69 | 6e 65 64 20 64 69 73 74 | determi|ned dist| |00005c30| 61 6e 63 65 20 28 31 33 | 20 69 73 20 74 68 65 20 |ance (13| is the | |00005c40| 64 65 66 61 75 6c 74 20 | 76 61 6c 75 65 29 20 74 |default |value) t| |00005c50| 68 61 74 20 63 61 6e 20 | 62 65 20 6d 6f 64 69 66 |hat can |be modif| |00005c60| 69 65 64 20 69 6e 20 74 | 68 65 20 69 74 65 6d 20 |ied in t|he item | |00005c70| 50 61 72 61 6d 65 74 65 | 72 73 20 6f 66 20 74 68 |Paramete|rs of th| |00005c80| 65 20 6d 65 6e 75 20 50 | 72 65 64 69 63 74 2e 20 |e menu P|redict. | |00005c90| 49 6e 20 74 68 61 74 20 | 63 61 73 65 2c 20 74 68 |In that |case, th| |00005ca0| 65 20 73 65 61 72 63 68 | 69 6e 67 20 77 69 6e 64 |e search|ing wind| |00005cb0| 6f 77 20 69 73 20 6c 69 | 6d 69 74 65 64 20 74 6f |ow is li|mited to| |00005cc0| 20 74 68 65 20 72 65 73 | 69 64 75 65 20 69 6d 6d | the res|idue imm| |00005cd0| 65 64 69 61 74 65 6c 79 | 20 62 65 66 6f 72 65 20 |ediately| before | |00005ce0| 74 68 65 20 66 69 72 73 | 74 20 47 6c 75 20 6f 72 |the firs|t Glu or| |00005cf0| 20 41 73 70 20 66 6f 75 | 6e 64 20 28 5a 6f 6e 65 | Asp fou|nd (Zone| |00005d00| 54 6f 20 70 61 72 61 6d | 65 74 65 72 29 2e 20 54 |To param|eter). T| |00005d10| 68 69 73 20 6c 69 6d 69 | 74 20 63 61 6e 20 62 65 |his limi|t can be| |00005d20| 20 75 73 65 64 20 69 6e | 73 74 65 61 64 20 6f 66 | used in|stead of| |00005d30| 20 74 68 65 20 64 65 66 | 61 75 6c 74 20 45 6e 64 | the def|ault End| |00005d40| 20 61 74 20 70 61 72 61 | 6d 65 74 65 72 2c 20 66 | at para|meter, f| |00005d50| 6f 6c 6c 6f 77 69 6e 67 | 20 74 68 65 20 50 72 65 |ollowing| the Pre| |00005d60| 66 65 72 65 6e 63 65 73 | 20 64 69 72 65 63 74 69 |ferences| directi| |00005d70| 76 65 73 20 28 73 65 65 | 20 61 62 6f 76 65 29 2e |ves (see| above).| |00005d80| 0d 0d 43 6c 65 61 76 61 | 67 65 20 73 69 74 65 0d |..Cleava|ge site.| |00005d90| 53 74 61 72 74 69 6e 67 | 20 61 74 20 74 68 65 20 |Starting| at the | |00005da0| 31 30 74 68 20 70 6f 73 | 69 74 69 6f 6e 20 61 6e |10th pos|ition an| |00005db0| 64 20 75 73 69 6e 67 20 | 61 6e 20 41 72 67 20 61 |d using |an Arg a| |00005dc0| 73 20 61 6e 63 68 6f 72 | 69 6e 67 20 70 6f 69 6e |s anchor|ing poin| |00005dd0| 74 2c 20 4d 69 74 6f 50 | 72 6f 74 20 6c 6f 6f 6b |t, MitoP|rot look| |00005de0| 73 20 66 6f 72 20 63 6c | 65 61 76 61 67 65 20 73 |s for cl|eavage s| |00005df0| 69 74 65 73 2e 20 54 68 | 65 20 66 69 72 73 74 20 |ites. Th|e first | |00005e00| 69 73 20 74 68 65 20 68 | 69 67 68 20 70 72 65 64 |is the h|igh pred| |00005e10| 69 63 74 69 76 65 20 52 | 2d 33 20 72 75 6c 65 20 |ictive R|-3 rule | |00005e20| 5b 6d 6f 74 69 66 20 52 | 58 59 28 53 2f 41 29 5d |[motif R|XY(S/A)]| |00005e30| 2e 20 49 66 20 69 74 20 | 64 6f 65 73 20 6e 6f 74 |. If it |does not| |00005e40| 20 65 78 69 73 74 2c 20 | 74 68 65 20 52 2d 31 30 | exist, |the R-10| |00005e50| 20 72 75 6c 65 20 5b 52 | 58 28 46 2f 49 2f 4c 29 | rule [R|X(F/I/L)| |00005e60| 53 5d 20 69 73 20 61 70 | 70 00 00 03 4c 6c 69 65 |S] is ap|p...Llie| |00005e70| 64 2e 20 49 66 20 6e 65 | 63 65 73 73 61 72 79 2c |d. If ne|cessary,| |00005e80| 20 74 68 65 20 52 2d 32 | 20 72 75 6c 65 20 28 52 | the R-2| rule (R| |00005e90| 58 58 53 29 20 69 73 20 | 61 6c 73 6f 20 75 73 65 |XXS) is |also use| |00005ea0| 64 2e 20 57 68 65 6e 20 | 74 68 65 20 52 2d 32 20 |d. When |the R-2 | |00005eb0| 61 6e 64 20 52 2d 31 30 | 20 72 75 6c 65 73 20 64 |and R-10| rules d| |00005ec0| 6f 20 6e 6f 74 20 6d 61 | 74 63 68 20 53 65 72 20 |o not ma|tch Ser | |00005ed0| 62 75 74 20 6d 61 74 63 | 68 20 6f 6e 65 20 6f 66 |but matc|h one of| |00005ee0| 20 74 68 65 20 73 6d 61 | 6c 6c 65 73 74 20 61 6d | the sma|llest am| |00005ef0| 69 6e 6f 20 61 63 69 64 | 73 20 28 47 6c 79 2c 20 |ino acid|s (Gly, | |00005f00| 41 6c 61 2c 20 53 65 72 | 2c 20 50 72 6f 2c 20 56 |Ala, Ser|, Pro, V| |00005f10| 61 6c 20 6f 72 20 54 68 | 72 29 2c 20 74 68 65 20 |al or Th|r), the | |00005f20| 73 69 74 65 20 69 73 20 | 71 75 61 6c 69 66 69 65 |site is |qualifie| |00005f30| 64 20 61 73 20 70 75 74 | 61 74 69 76 65 2e 20 43 |d as put|ative. 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Coef|20 refle| |00006300| 63 74 73 20 74 68 65 20 | 63 6f 6d 70 6f 73 69 74 |cts the |composit| |00006310| 69 6f 6e 20 6f 66 20 74 | 68 65 20 66 69 72 73 74 |ion of t|he first| |00006320| 20 32 30 20 73 65 71 75 | 65 6e 63 65 20 72 65 73 | 20 sequ|ence res| |00006330| 69 64 75 65 73 2e 20 4d | 54 53 20 73 68 6f 75 6c |idues. M|TS shoul| |00006340| 64 20 68 61 76 65 20 43 | 6f 65 66 32 30 20 3e 20 |d have C|oef20 > | |00006350| 30 2e 0d a5 20 43 6f 65 | 66 54 6f 74 20 77 68 69 |0... Coe|fTot whi| |00006360| 63 68 20 69 73 20 61 20 | 6d 6f 64 69 66 69 63 61 |ch is a |modifica| |00006370| 74 69 6f 6e 20 6f 66 20 | 43 6f 65 66 32 30 20 66 |tion of |Coef20 f| |00006380| 6f 72 20 74 61 6b 69 6e | 67 20 69 6e 74 6f 20 61 |or takin|g into a| |00006390| 63 63 6f 75 6e 74 20 6d | 6f 72 65 20 72 65 73 69 |ccount m|ore resi| |000063a0| 64 75 65 73 20 61 6e 64 | 20 74 68 65 20 6c 65 6e |dues and| the len| |000063b0| 67 74 68 20 63 6f 72 72 | 65 73 70 6f 6e 64 69 6e |gth corr|espondin| |000063c0| 67 20 74 6f 20 74 68 65 | 20 54 61 72 67 65 74 69 |g to the| Targeti| |000063d0| 6e 67 20 7a 6f 6e 65 20 | 28 5a 6f 6e 65 54 6f 20 |ng zone |(ZoneTo | |000063e0| 6c 65 6e 67 74 68 29 2e | 0d a5 20 46 72 6f 6d 20 |length).|.. From | |000063f0| 43 6f 65 66 32 30 20 61 | 6e 64 20 43 6f 65 66 54 |Coef20 a|nd CoefT| +--------+-------------------------+-------------------------+--------+--------+ Only 25.0 KB of data is shown above.